洞察前沿，连接科研新知

完整作者

Yuta Yamagishi、Yoshiki Sonoyama、Naoki Kawakami、Tomonori Hasebe、Masayuki Su'etsugu

作者机构

• Department of Life Science, College of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan.

技术路线

53 kb环状DNA模块基因组（编码26个RCR蛋白）→在PURE无细胞体系中一锅表达并自我复制→优化反应条件与解决表达瓶颈后实现递归自我复制（>28次倍增）

最近 5 个完整年度引用趋势

完整作者

Liujia Qian、Zizhuo Zhou、Peijie Zhou、Zhen Dong、Xudong Zhang、Zhenwu Dai、Zhangyang Gao、Siqi Sun、Kevin R Roy、Shuaiyao Wang、Nicola Zamboni、Charles Boone、Michael Costanzo、Jianzhe Li、Gianni Liti、Jia-Xing Yue、Markus Ralser、Evan Williams、Mattia Zampieri、Heng Jiang、Tailin Wu、Yalin Wang、Feiran Li、Joseph Schacherer、Rui Sun、Zhaoxing Li、Yaming Deng、Yi Chen、Zhiping Xie、Huiqiang Lou、Xiaowen Wang、Linhai Xie、Han Wen、Liangyi Chen、Kai Lei、George Rosenberger、Xue Cai、Yingrui Wang、Qi Xiao、Huaizong Shen、Gaowen Liu、Lei Ma、Brenda Andrews、Hui Lu、Kiryl Piatkevich、Yi Zhu、Lei Bai、Yizhi Cai、Yuping Chen、Weinan E、Ge Gao、Fuchu He、Luonan Chen、Stan Z Li、Hongwu Ma、Liang Qiao、Lars M Steinmetz、Leihan Tang、Tang Tang、Xiaofan Zhang、Jing Yang、Yifan Yang、Kaicheng Yu、Jianyang Zeng、Yefeng Zheng、Bowen Zhou、Tiannan Guo

作者机构

• Affiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, School of Future Biomedicine, Westlake University, Hangzhou, China.
• Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.
• Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, China.
• Center for Machine Learning Research, Peking University, Beijing, China.
• AI for Science Institute, Beijing, China.
• National Engineering Laboratory for Big Data Analysis and Applications, Beijing, China.
• Center for Data Science, Peking University, Beijing, China.
• Shanghai Artificial Intelligence Laboratory, Shanghai, China.
• Research Institute of Intelligent Complex Systems, Fudan University, Shanghai, China.
• Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA.
• Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
• Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
• The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
• Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
• Institute of Research on Cancer and Ageing of Nice (IRCAN), Faculté de Médecine, Nice, France.
• State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China.
• Department of Biochemistry, Charité-Universitätsmedizin Berlin, Berlin, Germany.
• Exploratory Diagnostic Sciences, Berlin Institute of Health at Charité, Berlin, Germany.
• Center for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
• Max Planck Institute for Molecular Genetics, Berlin, Germany.
• Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
• Department of Biomedicine, University of Basel, Basel, Switzerland.
• Department of Artificial Intelligence, School of Engineering, Westlake University, Hangzhou, China.
• Biomedical Research Core Facilities, Westlake University, Hangzhou, China.
• Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
• Université de Strasbourg, CNRS, Inserm, IGBMC UMR 7104-UMR-S 1258, Illkirch, France.
• State Key Laboratory of Microbial Metabolism and Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
• South China Hospital, Health Science Center, Guangdong Key Laboratory of Genome Instability and Disease Prevention, Shenzhen University School of Medicine, Shenzhen, China.
• State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics Driven Cancer Precision Medicine (Chinese Academy of Medical Sciences), Beijing, China.
• International Academy of Phronesis Medicine (Guangdong), Guangzhou, China.
• DP Technology Co. Ltd., Beijing, China.
• Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.
• State Key Laboratory of Medical Proteomics, Beijing, China.
• State Key Laboratory of Membrane Biology, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
• Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.
• Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China.
• Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, China.
• Bruker Switzerland AG, Faellanden, Switzerland.
• Zhejiang Key Laboratory of Structural Biology, School of Life Sciences, Westlake University, Hangzhou, China.
• Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
• National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing, China.
• State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
• SJTU-Yale Joint Center of Biostatistics and Data Science, National Center for Translational Medicine, MoE Key Lab of Artificial Intelligence, AI Institute Shanghai Jiao Tong University, Shanghai, China.
• Shanghai Engineering Research Center for Big Data in Pediatric Precision Medicine, NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology & Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai, China.
• School of Life Sciences, Westlake University, Hangzhou, China.
• Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China.
• Manchester Institute of Biotechnology, University of Manchester, Manchester, UK.
• Generative and Synthetic Genomics, Wellcome Sanger Institute, Cambridge, UK.
• Shenzhen Institute of Synthetic Biology, Chinese Academy of Sciences, Shenzhen, China.
• Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
• School of Mathematical Sciences, Peking University, Beijing, China.
• State Key Laboratory of Gene Function and Modulation Research, Biomedical Pioneering Innovative Center (BIOPIC) and Beijing Advanced Innovation Center for Genomics (ICG), School of Life Sciences, Center for Bioinformatics (CBI), Peking University, Beijing, China.
• School of Mathematical Sciences and School of AI, Shanghai Jiao Tong University, Shanghai, China.
• AI Laboratory, Research Center for Industries of the Future, Westlake University, Hangzhou, China.
• Biodesign Center, State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
• National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
• Department of Chemistry, Fudan University, Shanghai, China.
• Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
• DZHK (German Centre for Cardiovascular Research), Heidelberg, Germany.
• Stanford Genome Technology Center, Palo Alto, CA, USA.
• Center for Interdisciplinary Studies, Westlake University, Hangzhou, China.
• Wuhan Metware Biotechnology Co. Ltd., Wuhan, China.
• Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, China.
• State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Lifeomics, Beijing, China.
• School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, China.
• Autolab, Westlake University, Hangzhou, China.
• School of Engineering, Research Center for Industries of the Future, Westlake University, Hangzhou, China.
• Medical Artificial Intelligence Laboratory, Westlake University, Hangzhou, China.
• Department of Electronic Engineering, Tsinghua University, Beijing, China.
• Affiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, School of Future Biomedicine, Westlake University, Hangzhou, China. guotiannan@westlake.edu.cn.
• Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China. guotiannan@westlake.edu.cn.
• Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, China. guotiannan@westlake.edu.cn.

技术路线

多模态生物数据与机理知识、亚细胞结构、动态状态三类数据支柱 → 细胞复杂性分解为八个功能模块（遗传、代谢、结构系统），各模块为领域AI工具并由大语言模型调度层协调

最近 5 个完整年度引用趋势

完整作者

Daniel J Baker、Leon M Frommer、Ugur Uslu、Kisha K Patel、Daniel Zhu、Nils W Engel、James M George、Wencao Zhao、Samuel I Kim、Lisa Sun、Christopher Roselle、Philipp C Rommel、Regina M Young、Jonathan A Epstein、Sikander Hayat、Zoltan Arany、Carl H June

作者机构

• Center for Cellular Immunotherapies, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Cardiovascular Institute, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: bakerdj@upenn.edu.
• Department of Medicine 2, RWTH Aachen University, Medical Faculty, Aachen, Germany.
• Center for Cellular Immunotherapies, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Dermatology, Venereology and Allergology, University Hospital Würzburg, 97080 Würzburg, Germany.
• Center for Cellular Immunotherapies, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
• Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, MI, USA.
• Cardiovascular Institute, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
• Cardiovascular Institute, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA; Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA; Department of Cell and Developmental Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
• Department of Medicine 2, RWTH Aachen University, Medical Faculty, Aachen, Germany; Cardiovascular Research Institute, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Windreich Department of Artificial Intelligence and Human Health, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address: shayat@ukaachen.de.
• Cardiovascular Institute, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA; Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: zarany@upenn.edu.
• Center for Cellular Immunotherapies, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Parker Institute for Cancer Immunotherapy at University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: cjune@upenn.edu.

技术路线

单细胞RNA测序数据（人类皮肤癌与健康组织）整合→AI驱动候选靶点筛选，结合公共数据集优化肿瘤组成、组织特异性与临床可行性→大语言模型优先排序与提名→GPNMB跨血液与实体瘤表达验证→构建人源GPNMB CAR T细胞→单核细胞白血病、黑色素瘤和结直肠腺癌小鼠模型抗肿瘤活性验证

最近 5 个完整年度引用趋势

完整作者

Zahir Ali、Haroon Butt、Raghad Alghamdi、Jose Luis Moreno Ramirez、Magdy Mahfouz

作者机构

• Laboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
• Laboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia. magdy.mahfouz@kaust.edu.sa.

技术路线

样本：本氏烟草与水稻愈伤组织；分析：基于TMV游离型报告系统重建R2活性，检测28S rDNA阵列定点整合效率与保真度，并统计水稻rDNA抗性盒整合效率

最近 5 个完整年度引用趋势

完整作者

Javier M Hernández-Sancho、Daniel C Volke、Pablo I Nikel

作者机构

• BRIGHT, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.

技术路线

数据/样本：以Pseudomonas putida为宿主，构建基于Bxb1丝氨酸整合酶的级联双稳态开关，由鼠李糖诱导模块驱动，连接绝缘倒位模块产生二元OFF/ON转录状态；开关控制正交T7 RNA聚合酶基因以激活可替换的PT7靶模块；对整合酶与T7 RNA聚合酶基因进行翻译水平调谐。分析：评估开关效率、OFF态紧密性、诱导强度及去除诱导剂后的表型稳定性（遗传记忆）。验证：通过调控超活跃二鸟苷酸环化酶触发生物膜形成，并在2-氟顺,顺-黏康酸生产菌株中实现连续模式下开关介导的催化生物膜稳定产化。

最近 5 个完整年度引用趋势

完整作者

Xiaotong Wang、Jianping Xu、Yipeng Wang、Qingsheng Qi、Zhiguo Wang、Qian Wang

作者机构

• National Glycoengineering Research Center, Shandong University, Qingdao 266237, P. R. China.
• State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, P. R. China.
• Zhejiang Key Laboratory of Medical Epigenetics, Institute of Aging Research, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou 311121, P. R. China.

技术路线

数据/样本：20种sup-tRNA变体；分析：比较变体读通效率，归纳设计规则（反密码子与CUA相似性）；应用：LacI生物传感器、pykA/pykF调控的N-乙酰神经氨酸合成；验证：原文未报告独立正交性或脱靶验证

最近 5 个完整年度引用趋势

完整作者

Shengkun Tong、Yuxi Qin、Yaqian Sun、Yaojie Gao、Muyi Song、Tao Fang、Yaojun Tong

作者机构

• State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
• State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. yaojun.tong@sjtu.edu.cn.

技术路线

理性蛋白质工程（截短柔性区）构建减毒Cas13d变体 → 生长密度与转录本敲低评估

最近 5 个完整年度引用趋势

完整作者

Jun Hyung Sin、Christopher J Bowman、Joe Germino、Sloan H Phillips、Juliana Sucharov、Xian Liu、Yi Wang、Corey N Miller、Audrey V Parent、Aaron Bodansky、Mark S Anderson、Michael R Waterfield

作者机构

• Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA, USA.
• Department of Pediatrics, University of California, San Francisco, San Francisco, CA, USA.
• Department of Pathology, University of California, San Francisco, San Francisco, CA, USA.
• Diabetes Center, University of California, San Francisco, San Francisco, CA, USA.
• 10x Genomics, Pleasanton, CA, USA.
• Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
• Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA, USA. michael.waterfield@ucsf.edu.
• Department of Pediatrics, University of California, San Francisco, San Francisco, CA, USA. michael.waterfield@ucsf.edu.

技术路线

胸腺特异性Runx1敲除小鼠模型 → 胸腺mTEC群体转录/表型分析（鉴定表达表面活性剂基因的AT2样mTEC，并评估AIRE+ mTEC及T细胞选择）

最近 5 个完整年度引用趋势

完整作者

Juraj Gottweis、Wei-Hung Weng、Alexander Daryin、Tao Tu、Petar Sirkovic、Artiom Myaskovsky、Grzegorz Glowaty、Felix Weissenberger、Alessio Orlandi、Dan Popovici、Anil Palepu、Keran Rong、Ryutaro Tanno、Khaled Saab、Fan Zhang、Jacob Blum、Andrew Carroll、Kavita Kulkarni、Nenad Tomašev、Dina Zverinski、Ivor Rendulic、Elahe Vedadi、Florian Hasler、Luka Rimanic、Marina Boia、Ivan Budiselic、Ben Feinstein、Mathias Bellaiche、Tom Sheffer、Jan Freyberg、Jeremy Ratcliff、Ottavia Bertolli、Katherine Chou、Avinatan Hassidim、Burak Gokturk、Amin Vahdat、Yuan Guan、Vikram Dhillon、Eeshit Dhaval Vaishnav、Byron Lee、Tiago R D Costa、José R Penadés、Gary Peltz、Yossi Matias、James Manyika、Demis Hassabis、Yunhan Xu、Pushmeet Kohli、Annalisa Pawlosky、Alan Karthikesalingam、Vivek Natarajan

作者机构

• Google Cloud AI Research, Zurich, Switzerland. juro@google.com.
• Google DeepMind, Mountain View, CA, USA. ckbjimmy@google.com.
• Google Cloud AI Research, Zurich, Switzerland.
• Google DeepMind, Mountain View, CA, USA.
• Google Research, Mountain View, CA, USA.
• Stanford University School of Medicine, Palo Alto, CA, USA.
• Houston Methodist Hospital, Houston, TX, USA.
• Sequome, San Francisco, CA, USA.
• Fleming Initiative and Imperial College London, London, UK.
• Google DeepMind, Mountain View, CA, USA. pushmeet@google.com.
• Google Research, Mountain View, CA, USA. apawlosky@google.com.
• Google DeepMind, Mountain View, CA, USA. alankarthi@google.com.
• Google DeepMind, Mountain View, CA, USA. natviv@google.com.

技术路线

研究目标与科学证据输入→多智能体（基于Gemini）异步任务执行与锦标赛演化生成/批判/优化假设→自动化评估（测试时算力扩展）→三个生物医学应用（药物重定位、新靶点发现、抗菌素耐药机制解释）→急性髓系白血病候选药物与协同组合的体外实验验证

最近 5 个完整年度引用趋势

完整作者

Simran Gambhir、Shweta Pandey、Harsh Bajetha、Jasleen Kaur、Ankita Das、Gnanasekar Pranavathiyani、Rupali Aggarwal、Upasana Maity、Suhani Dange、Vipin Singh、Mayur Zarkar、Raghav Sankhdher、Bhupender Singh、Shivani Seth、Anshu Bhardwaj

作者机构

• Bioinformatics Centre, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
• Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
• UM-DAE Centre for Excellence in Basic Sciences, School of Biological Sciences, University of Mumbai Kalina Campus, Mumbai 400098, India.
• Life Sciences Department, J.C. Bose University of Science and Technology, YMCA, Sector 6, Mathura road, Faridabad Haryana 121006, India.
• Independent Researcher, IMTECH Housing, Sector 39A, Chandigarh 160036, India.

技术路线

数据/样本：聚焦WHO细菌优先病原体的参考基因组、泛基因组、AST谱、转录组数据集及多类注释资源（ARG、毒力因子、结构、宿主-病原互作、BGC、药物靶点等） → 分析：使用自建流程进行数据分析和注释，并按CLSI AST标准、EUCAST基因组序列指南及FAIR原则进行质量检查与人工整理

最近 5 个完整年度引用趋势